Some applications of this product may require a license which is not provided by the purchase of this product.
For research use only.
SolisFAST® Direct qPCR Kit (no ROX) has been engineered to bypass conventional DNA extraction and purification, combining rapid sample lysis, high tolerance to PCR inhibitors, and robust DNA amplification across diverse biological matrices, including plant materials (mung bean sprouts, soybeans, spinach, tomato, and lettuce leaves), fungal spores, whole blood, human oral buccal swabs, and mouse biopsy tissues.
With a streamlined lysis protocol requiring as little as 3 minutes, the kit enables efficient sample preparation for direct qPCR analysis. Target DNA input can be increased by using sample volumes of up to 50% of the total reaction volume while maintaining robust and reliable amplification performance.
10× Lysis Buffer enables efficient DNA release from eukaryotic and prokaryotic samples, simplifying sample preparation for direct qPCR analysis.
The resulting lysate can be used immediately or stored at −20 °C for over one month. It is fully compatible with the 5× SolisFAST® Direct qPCR Mix (no ROX), allowing reliable DNA amplification without additional purification.
5x SolisFAST® Direct qPCR Mix (no ROX) is a ready-to-use, 5× concentrated master mix optimized for rapid, highly sensitive, and reproducible probe-based qPCR. Compatible with dual-labelled hydrolysis probes (e.g., TaqMan® probes), it enables reliable detection and quantification of up to five targets simultaneously in a single multiplex reaction.
At its core is SolisFAST® DNA Polymerase, a novel, in silico-designed, inhibitor-tolerant variant of Taq DNA polymerase. Combining aptamer activation with an optimized reaction buffer, the mix delivers robust amplification performance and approximately 3-4-fold faster DNA extension rates compared with wild-type Taq DNA polymerase.
Sample type: Unprocessed biological material (tissue), purified DNA or cDNA
Polymerase activation: Aptamer based (3 minutes at 95 °C)
Product format: Kit
Cycling speed: fast
Targets: 1-5
Reference dye: None
Compatible real-time instruments: The mix is compatible with qPCR cyclers that do not require an internal reference dye (e.g. ROX) for normalization of fluorescent signal. Check Your cycler!
Just because the sample matrix is not in the list of validated matrices does not mean it cannot be successfully lysed with the 10x Lysis Buffer. For tailored recommendations, we suggest sending an email to our technical support (support@solisbiodyne.com), and we will provide suggestions on how to use the product specific to the sample matrix you are using.
Routine storage: -20 ºC
The kit can be stored at +4 °C for up to 6 months
Stability at room temperature (15-25 °C) for 1 month
Freeze-thaw stability: 30 cycles
At room temperature.
Universal Lysis Buffer in 10x concentration designed for rapid DNA extraction.
Ultrapure nuclease-free water for different molecular and chemistry purposes.
